今天阅读文献，发现了一批经实验验证的高效的miRNA引物，这些引物能特异扩充对应的96个与Cancer相关的miRNA，可以识别单碱基差异，其方法也比较独特，引物工作流程可以参考下图，具体实验流程可参考原文。对于用荧光定量PCR（RT-PCR）研究miRNA的研究人员或许有些用处。
| miRNA | RT primer | PCR reverse primer | PCR forward primer |
| hsa-let-7a | cgcatatcgcgtcattacagaaactatacaa | gcggagttgaggtagtaggttg | tcgcatatcgcgtcattacaga |
| hsa-let-7b | gcgacggagctacatatttgaaaccacaca | gtgactggtgaggtagtaggttg | tgcgacggagctacatatttga |
| hsa-let-7c | gtgcgagatcgttgtactgaaccatacaa | cgtcttcctgaggtagtaggttg | ctggtgcgagatcgttgtactg |
| hsa-let-7g | ggaggcgatataatcggctgactgtacaaa | agtgctgctttgaggtagtagttt | atggaggcgatataatcggctg |
| hsa-let-7i | gatgcccgttagtttgccacagcaca | gagagctgtgaggtagtagtttgt | gattgatgcccgttagtttgcc |
| hsa-mir-1 | ctcgacaaacgtctaaatgcttacatacttc | ccagtggagctggaatgtaaagaa | gctcgacaaacgtctaaatgct |
| hsa-mir-101 | cgcatgaatacgccaaacaacttcagttat | ccagcggttacagtactgtgata | ctcgcatgaatacgccaaacaa |
| hsa-mir-103 | cctggttcgatgtcctagagttcatagccc | ggttgacagcagcattgtacag | tcctggttcgatgtcctagagt |
| hsa-mir-106a | tgatgcagtctacgctgtcgctacctgc | atcccacaaaagtgcttacagtg | caatgatgcagtctacgctgtc |
| hsa-mir-106b | gtgcagtataggtacggctatctgcact | ccgcgtaaagtgctgacagt | atcgtgcagtataggtacggct |
| hsa-mir-107 | tggcgtttcgtcagagatgtgatagccc | gtcgtgagcagcattgtacag | tcttggcgtttcgtcagagatg |
| hsa-mir-10a | gcttgtcggttaaacactgtcacaaattcg | ccggtactaccctgtagatccg | aggcttgtcggttaaacactgt |
| hsa-mir-10b | gcacgagacttacggaggaacaaattcg | ttggagttaccctgtagaaccg | taagcacgagacttacggagga |
| hsa-mir-122a | cgcgtttaaccgagatttaccacaaacacc | catggcatggagtgtgacaatg | ccgcgtttaaccgagatttacc |
| hsa-mir-125a | cctcacaacgattccacaagcacaggtta | gttgattctccctgagacccttta | gtcctcacaacgattccacaag |
| hsa-mir-125b | gcaaccttgcgactataaccatcacaagtta | gtttcctctccctgagacccta | ggcaaccttgcgactataacca |
| hsa-mir-126 | gctcgacctcggaaactatggcattattac | agcatgaatcgtaccgtgagt | ctgctcgacctcggaaactatg |
| hsa-mir-126* | gcggaactttacgatacggcgcgtacc | ggcttcggcattattacttttggt | tacgcggaactttacgatacgg |
| hsa-mir-127 | gaaacacctgcgagattcacagccaagc | tttgttacatcggatccgtctga | tggaaacacctgcgagattcac |
| hsa-mir-128b | catccggctatgcgtattggaaagagacc | tggcgtcacagtgaaccg | cctcatccggctatgcgtattg |
| hsa-mir-129 | cgcgacatttagatgcgtggcaagccc | gtgacactttttgcggtctgg | caacgcgacatttagatgcgtg |
| hsa-mir-130a | gaggcgactgcctaaactatgcccttt | gccagccagtgcaatgttaaaa | tactgaggcgactgcctaaact |
| hsa-mir-133b | tccatcccgatcttctagcatagctggtt | cggagttattggtccccttcaa | catccatcccgatcttctagca |
| hsa-mir-135a | ccctgctcgcagtatttgagtcacatagga | gcggcagtatggctttttattcc | aaccctgctcgcagtatttgag |
| hsa-mir-135b | ctgccgctggaacaatttcacataggaa | cctcccgtttatggcttttcattc | ctaactgccgctggaacaattt |
| hsa-mir-136 | cgacccgcgacttactaatagtccatcatc | tgcagcactccatttgttttga | acgacccgcgacttactaatag |
| hsa-mir-137 | tcctcaggtcgaacctattgctacgcgt | ccgacgctattgcttaagaatacg | gctcctcaggtcgaacctattg |
| hsa-mir-140 | gatcgcacggacaactttcctaccatagg | gcaccgagtggttttaccct | ctagatcgcacggacaactttc |
| hsa-mir-141 | cgccaggataaattgacgcaccatctttac | ccgccttaacactgtctggta | atcgccaggataaattgacgca |
| hsa-mir-142-3p | cttgcatagtgcgcgtaataatccataaagt | ggaatccctgtagtgtttcctact | ccttgcatagtgcgcgtaataa |
| hsa-mir-143 | tcgtcgagataagctgtgtgtgagctaca | gttcgctgagatgaagcactg | gctcgtcgagataagctgtgtg |
| hsa-mir-145 | acttcgcaactaccgtttgaagggattc | gtaggaggtccagttttcccag | tgaacttcgcaactaccgtttg |
| hsa-mir-146a | ccgaatcttgccatacgcaaacccatgg | cggagtctgagaactgaattcca | taaccgaatcttgccatacgca |
| hsa-mir-146b | tgactcgcccataacgataaagcctatgg | tgaacgtgtgagaactgaattcca | gctgactcgcccataacgataa |
| hsa-mir-148a | ccgttcacgatccaaagacacaaagttct | cctcgcttcagtgcactacag | caaccgttcacgatccaaagac |
| hsa-mir-149 | cgtgattcgtgctcgtatatcggagtgaag | catcctttctggctccgtgt | gcgtgattcgtgctcgtatatc |
| hsa-mir-155 | ccagaaaccgatcagagtgtcccctatca | cgccatgtttaatgctaatcgtga | ttccagaaaccgatcagagtgt |
| hsa-mir-15a | ccctatcggttcgagacaccacaaacca | ggaatcctgtagcagcacataatg | ctaccctatcggttcgagacac |
| hsa-mir-16 | cctttgaggttggtactacggcgccaatat | gtgcagtagcagcacgtaaat | acctttgaggttggtactacgg |
| hsa-mir-17-3p | ggacttcgcccgataactacaagtgcc | agcttgaatttactgcagtgaagg | aagaggacttcgcccgataact |
| hsa-mir-17-5p | gccctccttccaacatagtactacctgc | accctccaaagtgcttacagtg | cttgccctccttccaacatagt |
| hsa-mir-181a | tgaacgtaactcgaatccgtactcaccg | cgtccaacattcaacgctgtc | cgtgaacgtaactcgaatccgt |
| hsa-mir-181b | acgacctctatgcggatgcccaccg | tgcactctaacattcattgctgtc | caagacgacctctatgcggatg |
| hsa-mir-183 | agctccgtagtcaggatgacacagtgaattc | gtacgctatggcactggtaga | agctccgtagtcaggatgaca |
| hsa-mir-18a | cgcgcctactttatgatggtatctgcac | aatcgcctaaggtgcatctagt | gtccgcgcctactttatgatgg |
| hsa-mir-191 | cctacgacgcagtaaatcacagctgctt | gaacacacaacggaatcccaaaa | tccctacgacgcagtaaatcac |
| hsa-mir-192 | gcgtatcaccgtgtttactgtggctgtca | gcccgtatctgacctatgaattga | agcgtatcaccgtgtttactgt |
| hsa-mir-193b | gactcagctcgtttgtgatgaaagcggg | aggtaaactggccctcaaagt | ctgactcagctcgtttgtgatg |
| hsa-mir-195 | gtgtaggcccaataccagagccaatattt | tgccacttagcagcacagaaa | ggagtgtaggcccaataccaga |
| hsa-mir-196a | gtcagaaggaatgatgcacagccaacaaca | acctgcgtaggtagtttcatgt | cgtcagaaggaatgatgcacag |
| hsa-mir-199a | aaggcgattgatacgagtcagaacaggta | ggtctccccagtgttcagacta | agaaggcgattgatacgagtca |
| hsa-mir-199a* | cccactcatagcgatttgctaaccaatgt | ggtagcctacagtagtctgcac | atcccactcatagcgatttgct |
| hsa-mir-19a | gataacggcaccgatctacttcagttttgc | agcctgtgtgcaaatctatgc | aggataacggcaccgatctact |
| hsa-mir-19b | ccctaccgcgcaataaacttcagttttgc | ccgctgtgcaaatccatgc | aatccctaccgcgcaataaact |
| hsa-mir-200a | acgccacaattaacaagccacatcgttac | gccgtctaacactgtctggta | cctacgccacaattaacaagcc |
| hsa-mir-200c | gctggcgaattagtagaccaccatcattac | ccaacgtaatactgccgggt | ctgctggcgaattagtagacca |
| hsa-mir-202 | agcctgtatcggaaattgctttttcccatg | acacgacttagaggtatagggca | ggagcctgtatcggaaattgct |
| hsa-mir-203 | aagcagtgatccggtctacctagtggtc | ggcgtaacgtgaaatgtttaggac | actaagcagtgatccggtctac |
| hsa-mir-204 | cacattagcgcgtattcagacaggcatagg | ccactttgattccctttgtcatcc | ccacattagcgcgtattcagac |
| hsa-mir-205 | tccgtcgttctaatgcgaacagactccg | cctccatccttcattccaccg | gtttccgtcgttctaatgcgaa |
| hsa-mir-206 | acgagtttagagccggatagccacacac | gcgttgtctggaatgtaaggaagt | tgacgagtttagagccggatag |
| hsa-mir-20a | ttcatttgttagcgagcggctacctgca | cgccatgtaaagtgcttatagtgc | cgattcatttgttagcgagcgg |
| hsa-mir-21 | cactgtctagcacgacactaatcaacatcag | gccaggcatagcttatcagactg | ccactgtctagcacgacactaa |
| hsa-mir-210 | cggtgtaggttcgtgattgactcagccgc | acgtactgtgcgtgtgaca | ccggtgtaggttcgtgattgac |
| hsa-mir-212 | gtcggaggaacgtagaagtggccgtg | ctcacgtaacagtctccagtca | atcgtcggaggaacgtagaagt |
| hsa-mir-214 | gctaaattcataaccggccaactgcctgt | actcacacagcaggcaca | cgctaaattcataaccggccaa |
| hsa-mir-215 | tccacgtcgatgatccctgtctgtcaa | gtcgccgtatgacctatgaattg | cctatccacgtcgatgatccct |
| hsa-mir-218 | ggtcgaacgcctaacgtcacatggttag | cgagtgcatttgtgcttgatcta | taatggtcgaacgcctaacgtc |
| hsa-mir-220 | ggaggctcggtattcttgttgaaagtgtcag | gctgaagccacaccgtatctg | aggaggctcggtattcttgttg |
| hsa-mir-221 | ccagtccacgatagtcccttgaaacccag | agtaggaaagctacattgtctgct | aaccagtccacgatagtccctt |
| hsa-mir-222 | ccacgacatagcggttacaagagacccag | tgacacaagctacatctggctac | gaccacgacatagcggttacaa |
| hsa-mir-223 | aggcgtcgagcttaatgtcggggtatttg | cgtagccctgtcagtttgtcaa | tctaggcgtcgagcttaatgtc |
| hsa-mir-224 | gtaagcacgctacatcctgataaacggaac | cgtttgccaagtcactagtggt | ttgtaagcacgctacatcctga |
| hsa-mir-24 | gcacatgactcgtagatacggctgttcctg | tgcgtggctcagttcagc | cgcacatgactcgtagatacgg |
| hsa-mir-25 | gcatactccgaccgttacttcagaccg | ggtgccattgcacttgtctc | tcagcatactccgaccgttact |
| hsa-mir-26a | accacacgtcatgtgactgcctatcct | agcgttgtttcaagtaatccagg | atcaaccacacgtcatgtgact |
| hsa-mir-26b | cttagcgttgcgatcttctcaacctatcct | ggccgcttcaagtaattcagg | tgcttagcgttgcgatcttctc |
| hsa-mir-27b | cggctaactaaccacgattctgcagaactt | ctttgtttccttcacagtggctaa | ccggctaactaaccacgattct |
| hsa-mir-29b | caagccctagtattcctcgacaacactgatt | agctccgtatagcaccatttgaaa | gcaagccctagtattcctcgac |
| hsa-mir-30a-5p | gcttgctcggtattagcctcttccagtc | agacaacgtgtaaacatcctcga | aatgcttgctcggtattagcct |
| hsa-mir-30b | gtcgagattgttgacgccagctgagtg | cggtgcattgtaaacatcctacac | gtatgtcgagattgttgacgcc |
| hsa-mir-30c | ttacggcgcttagtatcactgctgagagt | tggctccttgtaaacatcctacac | agttacggcgcttagtatcact |
| hsa-mir-31 | acgaacggcgtcatgtaacagctatgc | actccggcaagatgctgg | gagtacgaacggcgtcatgtaa |
| hsa-mir-32 | tctaccaaacgccaaacctgcaacttag | acggtggagtattgcacattacta | tgttctaccaaacgccaaacct |
| hsa-mir-328 | caccgtgtgcaattagaaggacggaagg | gctactggccctctctgc | ctcaccgtgtgcaattagaagg |
| hsa-mir-34a | gcagcgaatccacgattagaacaaccag | gaatggtggcagtgtcttagc | ggagcagcgaatccacgattag |
| hsa-mir-34c | cccatccctcactaagtgttggcaatcagc | tgcgaaggcagtgtagttagc | gcccatccctcactaagtgttg |
| hsa-mir-372 | tgactgcattcaagctcccacgctcaa | cgataggaaagtgctgcgacat | aagtgactgcattcaagctccc |
| hsa-mir-373 | ggcgattcaacgatctatcacacacccca | gctttcagaagtgcttcgattttg | cggcgattcaacgatctatcac |
| hsa-mir-376a | gccaagttaggtccattcgtacgtggatt | gcacagtgcatcatagaggaaaat | atgccaagttaggtccattcgt |
| hsa-mir-7 | agcattcgtctcgacacagcaacaaaatc | tgactctgctggaagactagtgat | tagagcattcgtctcgacacag |
| hsa-mir-9 | gttccaacccgactatctcatcatacagc | gccgtgtctttggttatctagc | tggttccaacccgactatctca |
| hsa-mir-92 | gccgtcgaggaatcctatcacaggccg | cgctcttattgcacttgtccc | gagccgtcgaggaatcctatca |
| hsa-mir-96 | cgtaccgagccttatcgtaagcaaaaatgt | ccgcttttggcactagcac | gtcgtaccgagccttatcgtaa |
| hsa-mir-98 | ggcgtaataatcgctccattcaacaatacaa | cgcaagaagtgaggtagtaagttg | cggcgtaataatcgctccattc |
| hsa-mir-99a | cgttggttgtcccatagactcacaagatc | ggcaaacccgtagatccga | tccgttggttgtcccatagact |